Motor neurons allow the body to move, speak, and breathe because of the propagation of action potentials between neuron nodes. The space between neurons, known as Ranvier’s node, is protected by a myelin sheath. When these nodes or sheaths are damaged, they destroy neuropathies such as schizophrenia, multiple sclerosis, Guillain-Barré syndrome, and many others. Of such importance, research aimed at the regrowth of the myelin sheath and the re-formation of Ranvier’s nodes for decades, although this research is timely and costly because human tissue samples, usually human brain tissue, have been further processed. is required to grow. Researchers at the University of Central Florida have developed a biomimetic, non-biological model for the formation of nodes of myelin and ranavier to study demyelinating diseases and test novel therapeutics. These models are based on the deposition of extracellular matrix proteins such as collagen on glass coverslips, which have been functionalized with UCT. Trimethoxysylpropyldiethylenetriamine (UCT Part# T2910), Stem cells and other neuron-based cells were incubated on these coverslips, and the researchers were able to confirm that both the myelin and nodules of ranvier formation did indeed occur. This novel model may reduce the time and cost of future research as there is no need to obtain complex samples such as brain tissue to study demyelinating disorders, but can instead be combined with induced pluripotent stem cells. which is easier to obtain blood or skin cells to replicate the disease outside the human body.
Citation: Patel, P.; Rumsey, JW; Lawrence, C.; Long, CJ; Lee, B.; Tetard, L.; Lambert, S.; Hickman, JJ, Myelination and node of ranvier formation in a human motoneuron-Schwann cell serum-free coculture, ACS Chem. neuroscience.2020, 11, 2615-2623.