In the pursuit of cancer research and clinical applications, one of the most important factors is the recognition and quantification of the CDKN2A/p16 anti-oncogene, AKA the multiple tumor suppressor 1 gene (MTS1). This gene is associated with the metastatic spread of tumor cells, which in general terms, the higher the amount of this gene, the worse the prognosis. Testing for this gene has faced many complications including laboriousness, low sensitivity, high cost and only semi-quantitative results, to name a few. Researchers, including a group in the Key Laboratory of Environmental Medicine and Engineering at Southeast University (Nanjing, China), have focused their attention on biosensing technologies that have high sensitivity, are cost-effective, and have been used to measure the MTS1 gene. can go. Turning to electrochemiluminescence (ECL) detection, which offers greater versatility and a more simplified setup, this team developed a method using electrospun nanofibers combined with core-shell luminescent composite nanoparticles to provide an enhanced sensitivity for quantification. Developed a biosensor device. This biosensor U. made using Tetraethoxysilane (T1807/T1808) to deposit silica nanoparticles on an electrode, and Trimethoxysilylpropyldiethylenetriamine (T2910) To actuate the electrodes first with glutaraldehyde, then single-stranded DNA which is finally combined with the luminescent core-shell nanoparticles. This biosensor demonstrated good biocompatibility, as well as high detection sensitivity with a wide linear range and good stability, comparable or even better than detection using other assays. This ECL biosensor is a promising tool for the detection of biomolecules at trace levels and will continue to be improved for future applications.
Citation: Wang, X., Wang, Y.; Shan, Y.; Jiang, M.; Gong, M.; Jin, X.; Wang, X.; Cheng, J., An electrochemiluminescence biosensor for CDKN2A/p16 anti-oncogene detection based on functionalized electrospun nanofibers and core-shell luminescent composite nanoparticles, Talenta2018, 187, 179-187.